It’s that time of year where wheat producers are in all stages of prep across the state, the graze out folks are wondering when the rains will hit to get the dusted in wheat up and the grain only folks are prepping grain drills or dealing with summer crop harvest. While this blog is focused on the wheat producers it should be acknowledged as a work of caution for anyone who pulls soil samples in environments where the temps during sampling can be above the mid 80’s.

Following some fantastic work by our Wild Cat Soil Fertility Counterparts (see blog) we dug into the same question of how does soil sample handling post collection impact the results. Dr. Ruiz-Diaz results on the impact of storage on nitrogen values was not surprising. But we wanted to go the next step and add in sample bag type.

Our Project

We collected a soil ground and homogenized. Placed in three types of bags Ziploc, Commercial (resin lined paper that is closed) and SWFAL bag (breathable material). Placed samples in a mini-van (Field) and brought a group onto campus (Office). With both sets of samples we had temperature loggers collecting hourly data. Every three days we collected four samples from each location determined moisture content and ran a full spectrum soil analysis including CO2 burst. The soil we used had a OM of 1.1% and soil pH of 6.1

Now let’s back up. Why was the KSU data not surprising. Well for those certified and honorary nitrogen ninjas we understand that the N-cycle processes of mineralization, immobilization, and nitrification are biological reactions which are significantly impacted by soil moisture and soil temperature. But the short and quick version is that the microbes that convert organic N to mineral N (NH4) and convert NH4 to NO3 like warm conditions with good soil moisture. So, when you pull soil samples, hopefully there is some moisture in the soil and then you place it in a bag and seal it. This creates an effective greenhouse environment where moisture cannot escape and is the perfect place for microbes to microbial things.  Therefore, you expect the organic cycle and nitrogen cycle to move and move quickly through the processes of mineralization, ammonification, and nitrification.

Back to the data.

First, we have the average daily temperatures from the sitting vehicle and the lab. On days 2-5 the average temp of the van was over 100°, after that the temps dropped. One thing to remember is the temp of the van is an average of a wide-ranging highs and lows between morning and midday while the office temperature being stable throughout the entire day.

What is the most interesting and revealing data may be the soil moisture of the samples. Unfortunately, day 3 samples went straight to the oven, but you can see by day 6 the SFWAL bags (yellow line) had dried significantly while it wasn’t until day 27 did the Commercial bags in the van (Black line w/ triangle) show signs of drying. It’s also important to note that while there are two lines (Field and Office) for the Commercial bags and Ziploc bags, but only one for the SWFAL bags. That is because for all variables measured there was never a significant difference between the SWFAL Field value and the SWFAL Office value.

We are going to start off with the variable that changed the most, NO³-N. This is also the form of N that is measured in most soil test. With a starting point of 3.4 lbs. N ac^-1 (based on a six inch soil sample depth) by day 6 the Field samples had jumped to 15.6 lbs. The office samples had much slower increase in NO3 with it taking 9 days to reach 10 lbs. You can also see that the error bars start showing up in the samples after about two weeks. The bars show the range in the sample results of a treatment. The interesting thing we saw was that the location within the van was significant. We set each replication of samples in a different spot in the van, the front seat, middle seat, and rear. The thermometers set with each replications showed that each area of the van had slightly differing temperature, which in turn affected the samples.

Ammonium (NH⁴-N), which is only reported by a few labs is also impacted by the storage location and bag type, however not to the sample level as NO3-N. Effectively the SWFAL bags and all samples taken to the office maintained consistent NH4 levels. The Com bag and Ziplocs left in the van however saw a significant increase in NH4 over time, basically a 2 lb per day increase.  

Of the other measured parameters such as potassium, sulfur, and CO₂ burst data were effected. These will be discussed more in depth in a peer reviewed publication and factsheet.

Take Home’s from the Work

While the majority of the nutrients were relatively un affected by the bag type or storage those that are more heavily influenced by organic matter and biological activity where, this includes many of the soil health parameters. The use of SWFAL soil bags resulted in data that was not impacted by storage or time.  While most clients of the OSU lab use these bags, more than one samples been brought into the county via a sealed Ziploc baggie. However the majority of the soil samples collected are placed into the resin lined paper bags and sealed. This data set is also important to those who use laboratories that run the Haney test which includes measures of NO₃, NH₄, Organic N, CO₂ respiration and H₂O extracted Organic C. It is critical that when using these type of bags that the samples be brought into climate controlled facilities as soon as possible. Its good to remember when looking at the data that the soil we used had a OM of 1.1%, initial NO₃ of 3.4 lbs. and NH₄ of 14 lbs. A soil with a higher OM% could have even greater change. Another viable is if the soil sample depth is greater than 6”. If your sample depth is 8” then the value is 33% higher.

Questions or comments feel free to reach out via email or social media.

Brian Arnall, Precision Nutrient Management b.arnall@okstate.edu