Brian Arnall, Oklahoma State University, Precision Nutrient Management Extension Specialist
Oliver Li, Oklahoma State University, Soil Chemistry

Interest in humic and fulvic acid products has increased substantially in agricultural production systems during the past two decades. These materials are frequently promoted as tools for improving soil biology, increasing nutrient availability, enhancing fertilizer efficiency, and stimulating plant growth. Because humic substances are known to be important components of soil organic matter, it is reasonable to ask whether adding humic or fulvic products to soil can meaningfully influence soil fertility.

As with many soil fertility questions, the answer depends on understanding two key factors: the mechanism involved and the magnitude of that mechanism relative to the soil system. Soil processes operate within large natural pools of organic matter, nutrients, and microbial activity. Therefore, evaluating the potential effects of humic products requires examining both how these compounds function chemically and biologically and how their application rates compare with the soils organic matter.

What Are Humic and Fulvic Acids?

Humic substances are heterogeneous organic compounds formed during the decomposition and transformation of plant and microbial residues. Historically, soil scientists have divided these materials into three operational fractions based on their solubility behavior: humic acid, fulvic acid, and humin (Stevenson, 1994; Tan, 2014). Humic acids are relatively large molecules that are insoluble under acidic conditions but dissolve in alkaline solutions. Fulvic acids are smaller molecules that remain soluble across the entire pH range, which allows them to move more freely in soil solution.

Both humic and fulvic acids contain numerous functional groups, particularly carboxyl and phenolic groups, which carry negative charge. These functional groups allow humic substances to interact with metal ions and nutrient cations and contribute to several important soil properties, including cation exchange capacity, buffering capacity, and metal complexation (Stevenson, 1994; Lehmann and Kleber, 2015). Because these materials originate from decomposed organic residues, they represent one portion of the complex mixture that collectively makes up soil organic matter. The distribution of the soil organic matter fractions varies among soil types and land uses, but fulvic acids and humic acids are each typically estimated to comprise approximately 10–35% of total soil organic matter (Guimarães et al., 2013).

Nutrient Retention and the Role of Cation Exchange

One of the most commonly cited mechanisms associated with humic substances is their ability to retain nutrients through cation exchange. The negatively charged functional groups present on humic molecules attract positively charged ions in soil solution. Through this electrostatic attraction, humic materials can retain several plant nutrients, including ammonium, potassium, calcium, magnesium, and certain micronutrients such as zinc and copper (Stevenson, 1994; Tan, 2014). This mechanism functions in the same manner as cation exchange on clay minerals. Of course, negatively charged surfaces do not retain negatively charged ions. As a result, nutrients such as nitrate are not held by humic substances and remain mobile in soil solution.

Laboratory measurements indicate that humic materials may possess relatively high cation exchange capacity on a mass basis. Reported values commonly range from approximately 300 to 600 cmolc kg⁻¹ depending on the source material and extraction method (Stevenson, 1994; Tan, 2014). These values demonstrate that humic substances can retain a large amount of cationic nutrients. A question that can be posed, however, is how this capacity compares with the nutrient retention already provided by soil organic matter.

Understanding the magnitude of humic additions requires comparing product application rates with the organic matter already present in soil. Calculations based on typical cation exchange values suggest that one pound of humic material with a CEC of 300–600 cmolc kg⁻¹ could theoretically retain approximately 0.04 to 0.08 pounds of ammonium-nitrogen. When viewed in isolation this number may appear meaningful. However, agricultural soils already contain large quantities of organic matter. An acre furrow slice, representing approximately the upper six inches of soil, weighs roughly two million pounds. Soil containing one percent organic matter therefore contains about 20,000 pounds of organic material per acre (Brady and Weil, 2016). Humified organic matter typically has cation exchange capacities ranging between 150 and 300 cmolc kg⁻¹ (Stevenson, 1994), meaning that the exchange capacity associated with native soil organic matter is already substantial. To put this into perspective, one pound of humic material can retain roughly 0.04 to 0.08 pounds of cation charge. Ammonium and potassium carry a single positive charge, while calcium carries two, meaning two ammoniums can be held for every two calcium. To provide contrast to the application of a humic substance, increasing soil organic matter by just 0.1% equivalent to about 2,000 pounds of additional organic material per acre can provide the capacity to retain approximately 40 to 80 pounds of cation charge or 40 to 80 pounds of ammonium.

The key point is not that humic materials cannot retain nutrients. They clearly can. Rather, the scale of material already present in soil is extremely large compared with the few ounces or pounds of humic products typically applied in agricultural systems. Consequently, the nutrient retention capacity associated with soil organic matter overwhelmingly dominates the soil system.

Micronutrient Complexation

Humic and fulvic substances are also known to interact with micronutrients through metal complexation reactions (also known as ‘chelation’). Carboxyl and phenolic functional groups can coordinate with metal ions such as iron, zinc, copper, and manganese to form organic complexes (Stevenson, 1994; Tan, 2014). These complexes can influence micronutrient mobility and availability in soils.

Fulvic acids are particularly effective at forming soluble complexes because they remain dissolved across the full range of soil pH. In some cases, these complexes may increase micronutrient mobility and transport within the soil solution. This mechanism has been well documented in soil chemistry research and may explain some responses observed in systems where micronutrient availability is limited.

Effects on Plant Physiology

In addition to soil chemical interactions, humic substances may influence plant growth through physiological mechanisms occurring in the rhizosphere. Several studies have shown that humic substances can stimulate root development, including increases in root elongation, lateral root formation, and root hair production (Nardi et al., 2002; Canellas and Olivares, 2014).

Research suggests that these responses may involve interactions with plant hormonal pathways and membrane transport processes. Humic substances have been shown to activate plasma membrane H⁺-ATPase enzymes, which are involved in proton pumping and nutrient uptake across root membranes (Canellas et al., 2002; Trevisan et al., 2010). Activation of these transport systems can enhance nutrient absorption and influence root architecture.

These physiological effects appear to occur primarily at the root–soil interface, where dissolved organic molecules interact directly with plant tissues. As a result, the responses observed in plant growth experiments are often attributed to rhizosphere signaling processes rather than large changes in bulk soil fertility.

Microbial Responses to Humic and Fulvic Compounds

Soil microorganisms respond strongly to carbon availability, and different carbon sources can produce very different microbial responses. Simple carbohydrates such as glucose and sucrose are readily metabolized by soil microbes and therefore produce rapid increases in microbial respiration and biomass. Humic substances, in contrast, consist of chemically complex and partially oxidized organic compounds that decompose much more slowly (Lehmann and Kleber, 2015).

Experimental studies comparing carbon sources consistently show that microbial respiration increases dramatically when simple sugars are added to soil, whereas humic substances produce smaller responses (Blagodatskaya and Kuzyakov, 2008). This difference reflects the relative degradability of these compounds as microbial energy sources.

Carbon Inputs from Humic Products Compared with Natural Soil Carbon

Soil microbial activity is largely driven by carbon supplied from plants through root exudation, residue decomposition, and organic matter turnover. The carbon pools already present in soil are therefore important for understanding the potential influence of humic product additions. A soil containing one percent organic matter holds approximately 11,600 pounds of carbon per acre (Brady and Weil, 2016).

Research on plant–soil carbon cycling indicates that living roots release significant quantities of organic carbon into soil each growing season through root exudation and rhizodeposition (Kuzyakov and Domanski, 2000). These plant-derived carbon inputs commonly amount to hundreds of pounds of carbon per acre and serve as a major energy source for soil microbial communities. Viewed in this context, humic product applications represent extremely small additions to the soil carbon pool. Consequently, microbial stimulation in agricultural soils is dominated by carbon inputs from plant residues and root exudates rather than by small additions of humic materials.

Building Organic Matter in the Central Plains

Increasing soil OM in the central Great Plains is achievable, but the magnitude of change is governed primarily by carbon inputs and water availability rather than any single management practice. Systems that combine no-till, increased residue return, diversified crop rotations, and where feasible cover crops or manure inputs are the most effective because they simultaneously increase carbon inputs and reduce decomposition losses (Lyon et al., 2007; Mikha et al., 2013; Nielsen et al., 2016). In semi-arid systems, realistic rates of OM increase are modest: over a 5-year period, changes are often small, approximately +0.05 to 0.1% OM, but significant in relation to the system which is often at total OM levels between 0.7 and 1.25 prior to establishment of conservation practices. The increase is confined to the top inch of the soil surface (Mikha et al., 2013; Saha et al., 2024). Mechanistically, these gains occur through greater residue and root-derived carbon inputs, reduced soil disturbance which slows microbial oxidation, and improved aggregation that physically protects organic matter from decomposition (Six et al., 2002; Lehmann and Kleber, 2015). However, as emphasized throughout this discussion, the scale of change is small relative to the large existing organic matter pool, and meaningful increases require long-term, system-level management focused on maximizing biomass production rather than relying on small external carbon additions such as commercial products.

Take-Home Points

• Humic and fulvic acids can retain cations, chelate micronutrients, and influence plant and microbial processes.
• Typical application rates are small relative to existing soil organic matter, so whole-soil impacts are limited.
• Most observed effects are localized in the rhizosphere, not broad changes in soil fertility.
• Evaluating both mechanism and scale is key to understanding their role in nutrient management.

References

Blagodatskaya, E., & Kuzyakov, Y. (2008). Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure. Biology and Fertility of Soils, 45(2), 115–131.

Brady, N. C., & Weil, R. R. (2016). The nature and properties of soils (15th ed.). Pearson.

Canellas, L. P., Olivares, F. L., Okorokova-Façanha, A. L., & Façanha, A. R. (2002). Humic acids isolated from earthworm compost enhance root elongation and lateral root emergence in maize. Plant Physiology, 130(4), 1951–1957.

Canellas, L. P., & Olivares, F. L. (2014). Physiological responses to humic substances as plant growth promoters. Chemical and Biological Technologies in Agriculture, 1, 3.

Guimarães, D. V., Gonzaga, M. I. S., Silva, T. O., Silva, T. L., Dias, N. S., & Matias, M. I. S. (2013). Soil organic matter pools and carbon fractions in soil under different land uses. Soil and Tillage Research, 126, 177–182.

Kuzyakov, Y., & Domanski, G. (2000). Carbon input by plants into the soil: Review. Journal of Plant Nutrition and Soil Science, 163(4), 421–431.

Lehmann, J., & Kleber, M. (2015). The contentious nature of soil organic matter. Nature, 528(7580), 60–68.

Lovley, D. R., Coates, J. D., Blunt-Harris, E. L., Phillips, E. J. P., & Woodward, J. C. (1996). Humic substances as electron acceptors for microbial respiration. Nature, 382, 445–448.

Lyon, D. J., Stroup, W. W., & Brown, R. E. (2007). Crop production and soil water storage in long-term winter wheat–fallow tillage experiments. Soil and Tillage Research, 94(2), 387–397.

Mikha, M. M., Vigil, M. F., Benjamin, J. G., & Sauer, T. J. (2013). Cropping system influences on soil carbon and nitrogen stocks in the Central Great Plains. Soil Science Society of America Journal, 77(2), 702–710.

Nardi, S., Pizzeghello, D., Muscolo, A., & Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry, 34(11), 1527–1536.

Nielsen, D. C., Lyon, D. J., Hergert, G. W., Higgins, R. K., Calderón, F. J., & Vigil, M. F. (2016). Cover crop mixtures do not use water differently than single-species plantings. Agronomy Journal, 108(3), 1025–1038.

Saha, D., Kukal, S. S., & Bawa, S. S. (2024). Long-term impacts of conservation agriculture practices on soil organic carbon and aggregation. Soil Science Society of America Journal.

Six, J., Conant, R. T., Paul, E. A., & Paustian, K. (2002). Stabilization mechanisms of soil organic matter: Implications for C saturation of soils. Plant and Soil, 241(2), 155–176.

Stevenson, F. J. (1994). Humus chemistry: Genesis, composition, reactions (2nd ed.). Wiley.

Tan, K. H. (2014). Humic matter in soil and the environment. CRC Press.

Trevisan, S., Francioso, O., Quaggiotti, S., & Nardi, S. (2010). Humic substances biological activity at the plant–soil interface. Plant Signaling & Behavior, 5(6), 635–643.

For any questions or commments please feel free to reach out to Brian Anrall, b.arnall@okstate.edu